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KMID : 0545120100200010193
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Journal of Microbiology and Biotechnology
2010 Volume.20 No. 1 p.193 ~ p.201
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A Real-time PCR assay for the quantitative detection of Ralstonia solanacearum in the horticultural soil and plant tissues
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Chen Yun
Zhang Wen-Zhi
Li Bo
Guo Jian-Hua
Liu Xin
Ma Zhong-Hua
Allen Caitilyn
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Abstract
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A specific and rapid real-time PCR assay for detecting Ralstonia solanacearum in the horticultural soil and plant tissues was developed in this study. The specific primers RSF/RSR were designed based on the upstream region of UDP-3-O-acyl-GlcNAc deacetylase gene from R. solanacearum, and a PCR product of 159 bp was amplified specifically from 28 strains of R. solanacearum, which represent all genetically diverse AluI types and all 6 biovars, but not from any other nontarget species. The detection limit of 102 CFU/g tomato stem and horticultural soil was achieved in this real-time PCR assay. The high sensitivity and specificity observed with filed samples as well as with artificially infected samples suggested that this method might be a useful tool for detection and quantification of R. solanacearum in precise forecast and diagnosis.
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KEYWORD
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Detection, Quantification, Ralstonia solanacearum, Real-time PCR
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